Photocatalysis-enhanced synthesis and stabilization of silver nanoparticles by methanol-based phytochemicals extract of Trigonella foenum-graecum seeds

Abstract

Trigonella foenum-graecum is an economically important plant that has significant nutraceutical properties. Various parts of the plant have previously been reported to synthesize metal nanoparticles. However, the seeds of the plant have limited potential to synthesize metal nanoparticles. Green synthesis of silver nanoparticles requires phytochemicals as reducing and metal chelating agents, in addition to the stabilising agents that play critical role in nanoparticles stabilization. The quantitative analysis of the methanol extract of the seeds suggest that the extract has significant antioxidant activity and reducing potential which is comparable to that of ascorbic acid. Likewise, GCMS data of the extract identified several phytochemical components that have nanoparticles stabilizing potential. Evidently, the extract indeed synthesized silver nanoparticles in dark, albeit in very low quantity. This limitation of low quantity of nanoparticles synthesis was overcome by photocatalysis. The rate of nanoparticles synthesis increased significantly with increase in the intensity of the white light-emitting diode (LED) light. Furthermore, the photocatalytic effect of the white light also has significant impact on the physicochemical characterisation of the nanoparticles. Particle size, nanoparticles yield and elemental analysis demonstrated that the 2000 lumens white LED light is optimum for photocatalysis as compared to the 250 lumens and 825 lumens light. However, the stability of nanoparticles is not influenced by photoirradiation, and is rather controlled by the phytochemical composition of the extract. Methanol extract of the seeds significantly enhanced the stability of the silver nanoparticles irrespective of the light intensities used for photocatalysis.