Abstract
Use of mesenchymal stem cells and low-level laser therapy (LLLT) have been widely studied to promote bone healing. evaluate effect of photobiomodulation on total number of cells (TNC) and cell viability (CV) of fresh bone marrow aspirate (BMA). Femur BMA from 10 adult rats was collected and a cell concentration of 1x107 cell/mL was obtained. Cell suspension was deposited on 96 well cell culture plates and distributed in groups: 1) RPMI, positive control; 2) Distilled Water, negative control; 3) Red Laser (RL); 4) Infrared Laser (IRL). Groups RL and IRL received LLLT application right after incubation. Cells were incubated for 24 h. TNC and CV were assessed through trypan blue assay after 1, 3, 6, 10 and 24 h of incubation. Data distribution was verified by Shapiro-Wilk test. Kruskal-Wallis test was used for intergroup and intragroup comparisons (p<0.05). TNC: after 1 and 3 h, groups RL and IRL presented significantly higher TNC than Group Water; after 6 and 10 h, groups RPMI, RL and IRL presented significantly higher TNC than Group Water. CV: after 1 h, groups RL and IRL showed significantly higher percentage of VC than Group Water; after 3, 6 and 10 h, all groups presented significantly higher percentage of VC than Group Water. It can be concluded that LLLT enhanced number and viability of fresh bone marrow aspirate cells.
Highlights
Development of biological substitutes for tissue restoration from cell transplantation have been studied by tissue engineering, the major components of regenerative medicine (Zambon et al, 2020)
The present study was delineated from the promising results observed in previous studies (Santinoni et al, 2020; Nagata et al, 2013) where the combination of bone marrow aspirate (BMA) and LLLT demonstrated benefits over the repair of critical bone defects surgically created in rat calvaria
In order to better evaluate the effect of LLLT on bone marrow cells, total number of cells and cell viability rate were assessed through the trypan blue assay
Summary
Development of biological substitutes for tissue restoration from cell transplantation have been studied by tissue engineering, the major components of regenerative medicine (Zambon et al, 2020). Studies have evaluated mesenchymal stem cell therapy (MSC) derived from bone marrow with purpose of restore and maintain tissue repair (Burastero et al, 2010; Schneider et al, 2010). Cultivation of MSC is a promising approach, but in vitro processing causes several changes in the phenotype of these cells, which may influence regenerative therapies results (Bara et al, 2014). These phenotypic modifications concerning in vitro cultivation of CTM have led researchers to find alternatives such as the use of unprocessed bone marrow or isolated mononuclear cells (Bara et al, 2014). Transplantation of autogenous MSC to the site of tissue injury is a promising tool for cell-based strategies necessary for bone regeneration, tissue repair and healing (Smiler et al, 2008)
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