Abstract
Time-resolved infrared (IR) spectroscopy is a well established technique for the study of photobiological systems. In this review attention is focused on technical improvements (instrumental, data analysis, spectral simulation, methodology, sample preparation, isotope-labelling strategies, vibrational probes) and on the scientific results obtained since 2015 for time-resolved IR spectroscopy in the (sub-) ns – second time range. A peculiar emphasis will be given to studies in the field of photosynthesis. Finally, after the general conclusions, the perspectives for the application of time-resolved IR spectroscopy in photobiology are described.
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