Abstract
`HanaQueen' tomato plantlets were cultured under conditions with different levels of sugar, photosynthetic photon flux, CO2 concentration, and number of air exchanges of the vessel. Effects of medium substrates (Gelrite or vermiculite) and explant preparation (with or without leaves) on growth of the plantlets were also examined. After 20 days in culture, photoautotrophically cultured plantlets with leafy explants, under increased PPF, CO2, and ventilation rate of the vessel had twice as much dry weight as those cultured conventionally with non-leafy explants under low PPF, CO2, and ventilation rate of the vessel. Dry weight of the plantlets was significantly greater when cultured with leafy than non-leafy explants. Net photosynthetic rate of the plantlets increased linearly as culture period when cultured without sugar, and remained almost zero when cultured with sugar, regardless of other culture conditions. Results obtained in this experiment have shown that tomato plantlets can be grown photoautotrophically, and the net photosynthetic rate was greater under photoautotrophic than under conventional photomixotrophic conditions.
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