Abstract
Photo-responsive cell attachment surfaces can simplify patterning and recovery of cells in microdevices for medicinal and pharmaceutical research. We developed a photo-responsive surface for controlling the attachment and release of adherent cells on a substrate under light-guidance. The surface comprises a poly(ethylene glycol) (PEG)-based photocleavable material that can conjugate with cell-adhesive peptides. Surface-bound peptides were released by photocleavage in the light-exposed region, where the cell attachment was subsequently suppressed by the exposed PEG. Simultaneously, cells selectively adhered to the peptide surface at the unexposed microscale region. After culture, the adhered and spread cells were released by exposure to a light with nontoxic dose level. Thus, the present surface can easily create both cell-adhesive and non-cell-adhesive regions on the substrate by single irradiation of the light pattern, and the adhered cells were selectively released from the light-exposed region on the cell micropattern without damage. This study shows that the photo-responsive surface can serve as a facile platform for the remote-control of patterning and recovery of adherent cells in microdevices.
Highlights
Microdevices for cell analysis and cell manipulation are being developed for various fields, from fundamental cell biology research to regenerative medicine [1]
The surface was designed to display the maleimide moiety as the reactive group for tethering the RGD peptide via the photolabile linker on the poly(ethylene glycol) (PEG) monolayer (Figure 1)
The molecular design of RGD peptides with a cysteine residue at the N-terminus allows for conjugation with a maleimide moiety via a Michael reaction, and under exposure to light, the peptides detach from the PEG monolayer through the photocleavage of the linker (Figure 1, left bottom)
Summary
Microdevices for cell analysis and cell manipulation are being developed for various fields, from fundamental cell biology research to regenerative medicine [1]. Development of materials for photo-responsive surfaces that achieves both light-guided patterning and recovery of living cells is very important for a variety of practical uses. To assay the native phenotype of adhered cells, a substrate surface was coated with collagen as a scaffold for cellular adhesion, followed by modification of the collagen coating with PEG-lipid at low density [20] The adhered cells are readily released from the desired region of the cell micropattern by exposure to non-cytotoxic light Both the cell attachment and release on the surface in response to light were examined, and light-guided patterning and recovery of cells were conducted to show the usability of the surface (Figure 1).
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