Abstract
A new stationary phase for high-performance liquid chromatography was produced by reacting a conventional amino bonded phase with phosphoric acid. Characterization of the modified amino phase indicated that ammonium phosphate salt groups and “free” phosphoric acid were present on the stationary phase surface. The column's capability for separating complex sample mixtures into chemical classes was tested using model compounds representing those typically found in fossil fuels. A solvent gradient program was developed which effectively separated hydrocarbon standards into aliphatic, non-polar aromatic, neutral/acidic polar aromatic, and basic polar aromatic classes.
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