Abstract

This chapter provides an overview of neurofilament structure and phosphorylation, with major emphasis on protein kinases and protein phosphatases involved in the modification of neurofilament proteins. The chapter shows that treatment of cultured dorsal root ganglion neurons with okadaic acid caused a shift in the electrophoretic mobilities of neurofilament subunits, signifying their increased phosphorylation. This was accompanied by the ordered conversion of neurofilament subunits to Triton X- 100-soluble forms and by marked changes in their immunofluorescent staining patterns. These results indicate that neurofilaments are dynamic entities whose assembly and organization are modulated by the balanced addition and removal of phosphate moieties.

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