Phosphorus confers tolerance against manganese toxicity in Prunus persica by reducing oxidative stress and improving chloroplast ultrastructure

Abstract

In this study, we evaluated the mitigative role of phosphorus (P) in terms of manganese (Mn) toxicity in peach (Prunus persica L.) plants. Ten-day-old seedlings were treated with excess Mn (1 mM MnSO4) alone and in combination with different P levels (100, 150, 200 and 250 μM KH2PO4) in half-strength Hoagland medium. The results demonstrated that Mn toxicity plants accumulated a significant amount of Mn in their tissues, and the concentration was higher in roots than in leaves. The accumulated Mn led to a considerable reduction in plant biomass, water status, chlorophyll content, photosynthetic rate, and disrupted the chloroplast ultrastructure by increasing oxidative stress (H2O2 and O2•−). However, P supplementation dramatically improved plant biomass, leaf relative water and chlorophyll contents, upregulating the ascorbate-glutathione pool and increasing the activities of antioxidant enzymes (superoxide dismutase; peroxidase dismutase; ascorbate peroxidase; monodehydroascorbate reductase; dehydroascorbate reductase), thus reducing oxidative damage as evidenced by lowering H2O2 and O2•− staining intensity. Moreover, P application markedly restored stomatal aperture and improved chloroplast ultrastructure, as indicated by the improved performance of photosynthetic machinery. Altogether, our findings suggest that P (250 μM) has a great potential to induce tolerance against Mn toxicity by limiting Mn accumulation in tissues, upregulating antioxidant defense mechanisms, alleviating oxidative damage, improving chloroplast ultrastructure and photosynthetic performance in peach plants.