Abstract
1. 1. A strip-transfer technique, which enables phospholipids to be eluted from one type of paper on to a second type of paper, has resulted in improved resolution of phospholipid mixtures from yeast by two-dimensional paper chromatography. The most satisfactory separations were obtained when lipid mixtures were chromatographed in the first direction on formaldehyde-treated, alumina-impregnated, or anion exchange paper, followed by elution on to silicic acid-impregnated paper and development in the second direction. Resolution of lipid mixtures was less satisfactory when chromatography was carried out in the first direction on alumina- or silicic acid-impregnated paper, followed by development on to formaldehyde paper in the second direction. The merits of the various systems are discussed and a simple apparatus is described which facilitates the strip-transfer procedure. 2. 2. The Chromatographie methods described are particularly effective for detecting minor phospholipids (phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine and N, N-dimethylphosphatidylethanolamine) in crude lipid extracts.
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