Abstract
Phospholipid (PL) fatty acid composition and stereospecific distribution of 25 genetically modified soybean lines having a wide range of compositions were determined by gas chromatography and phospholipase Aj hydrolysis. PL contained an average of 55.3% phosphatidylcholine, 26.3% phosphatidylethanolamine and 18.4% phosphatidylinositol, PL class proportions were affected by changes in overall fatty acid composition. PL fatty acid composition changed with oil fatty acid modification, especially for palmitate, stearate and linolenate. Stereospecific analysis showed that saturated fatty acids were primarily located at the sn-1 position of all PLs, and changes of the saturates in PLs were largely reflected on this position. Oleate was distributed relatively equally between the sn-1 and sn-2 positions. Linoleate was much more concentrated on sn-2 than on sn-1 position for all PLs. Linolenate was distributed relatively equally at low concentration but preferred the sn-2 position at high concentration. ' Reprinted with permission from J. Am. Oil Chem. Soc. 74(12);1587-1594 (1997), copyright© 1997. ^ Department of Food Science and Human Nutrition and the Center for Crops Utilization Research. ^ Author for correspondence. ^ Department of Agronomy.
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