Abstract
Phospholipase C-δ1 Expression Is Linked to Proliferation, DNA Synthesis, and Cyclin E Levels
Highlights
We previously reported that phospholipase C-␦1 (PLC-␦1) accumulates in the nucleus at the G1/S transition, which is largely dependent on its binding to phosphatidylinositol 4,5bisphosphate
Transient Expression of Human PLC␦1 Reduces Levels of Cyclin E in Cells Treated with small interfering RNA (siRNA) and Synchronized to the
In an attempt to explain this phenomenon, we investigated whether siRNA-mediated suppression of PLC␦1 altered key regulators of S phase progression [37, 38]
Summary
We previously reported that phospholipase C-␦1 (PLC-␦1) accumulates in the nucleus at the G1/S transition, which is largely dependent on its binding to phosphatidylinositol 4,5bisphosphate We find that suppression of PLC␦1 increases cyclin E levels, alters S phase progression, and inhibits cell proliferation. No significant changes were found among cultures in their G1-DNA or S phase DNA content, analysis indicated a significant decrease in the number of G2/M-DNA content cells compared with control conditions (Fig. 3, inset), suggesting a delay in S phase progression.
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