Abstract
Friend erythroleukemia cells have a nuclear phosphoinositide cycle which is related to both mitogen-stimulated cell growth and erythorid differentiation. Because of the important role of the phosphatidylinositol-transfer protein (PI-TP) in phosphatidylinositol 4,5-bisphosphate (PtdInsP2) synthesis, we have analysed nuclei isolated from Friend cells for the presence of PI-TP. By Western Blotting it was demonstrated that both intact nuclei and nuclei deprived of the outer membrane contained the PI-TPα isoform. Upon induction of erythroid differentiation by DMSO, the amount of nuclear PI-TPα was greatly diminished. As shown previously, under these same conditions, nuclear phospholipase Cβ1(PLCβ1) is down-regulated as well.
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