Abstract
Friend virus transformed erythroid cells can be isolated and maintained serially in cell culture [1,2]. Such erythroleukemic cell cultures can be induced to differentiate along the erythroid pathway by exposure to a variety of compounds including DMSO [1–3], aprotic solvents [4], fatty acids [5], purines and purine analogs [6] and the cardiac glycoside ouabain [7]. Phenotypic changes observed in Friend cells after inducer treatment appear to parallel the expression of genes typical of the normal differentiating cell. A multitude of erythroid functions are induced including the induction of heme pathway enzymes [8]. globin mRNA synthesis [2,3,9,10], and the synthesis of erythrocyte specific proteins [11–13]. The extent of hemoglobin synthesis can be as high as 20–25% of the total cellular protein synthesis in induced cells, compared to less than 1% in uninduced cells.KeywordsAmino Acid TransportAmino Acid UptakeErythroid DifferentiationErythroleukemic CellSustained InhibitionThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Published Version
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