Phosphoglycolate phosphatase: Immunological comparisons of the enzyme from the different photosynthetic groups of plants using maize polyclonal antiserum

Abstract

The immunochemical relationship of Zea mays (L.) phosphoglycolate phosphatase to that of higher plants chosen from different morphological (monocotyledons, dicotyledons) and photosynthetic types (C 3, C 3−C 4, C 4, CAM) was determined by Oucherlony double diffusion analysis using a rabbit polyclonal antiserum. The crude leaf extracts of Hordeum vulgare (L.) Triticum aestivum (L.), Panicum bisulcatum (Thumb.), Panicum milioides (Nees ex-Trin), Panicum maximum (Jacq.), Panicum miliaceum (L.), Sorghum bicolor (L.) Amaranthus caudatus (L.), Glycine max (L.), Phaseolus vulgaris (L.), Helianthus annuus (L.) and Kalanchoe daigremontiana (Hamet and Perier), cross-reacted with maize phosphoglycolate phosphatase antiserum indicating a serological relationship. A complete immunochemical identity between the extracts of monocotyledonous species, chosen among the 3 photosynthetic groups of plants, was revealed by the complete fusion of the immunoprecipitin bands. However, the immunological comparison between the crude extracts firstly from some C 3 and C 4 dicotyledonous species, secondly from dicotyledonous (C 3, C 4) and monocotyledonous (C 3, C 3−C 4 and C 4) species and finally between the crude extracts from Kalanchoe, an obligatory CAM, and all other plants investigated in this work, conducted to a partial continuity of the immunoprecipitin bands. These results indicate a serological relationship between these phosphoglycolate phosphatases that are not immunochemically identical. Moreover, the spur formation seen on the precipitation patterns means that the enzyme from the C 3 and C 4 dicotyledonous species investigated contained fewer cross-reacting determinants than the Kalanchoe protein when the anti-maize phosphoglycolate phosphatase immunoserum was used.