Abstract
Summary Specific polyclonal antibodies raised against corn phosphoglycolate phosphatase were used for immunodetection of this protein in the two chlorophyllous cells, mesophyll protoplasts and bundle sheath strands, isolated enzymatically from expanded corn leaves. Western blot analysis clearly showed an immunoreactive protein band with the bundle sheath cells only; its molecular mass of 31.5 kilodaltons corresponds to the phosphoglycolate phosphatase subunit. This result is totally in accordance with the cellular localization of this specific phosphatase activity, previously shown in bundle sheath cells only, and means that phosphoglycolate phosphatase gene expression was different in the two photosynthetic cell types of maize leaves, a C 4 plant.
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