Abstract
Research on the exploration and identification of phosphate solvent fungi has never been carried out in West Bali National Park (TNBB), therefore researchers aims to explore and identify microscopic fungi to be used as phosphate solvent fungi which later will be taken from each plant soil samples (Lantana camara) that the presence is very common in TNBB. The research was implemented in two stages. The first stage is exploration of soil fungi in the field (TNBB) and identification of fungal species and the second stage is the phosphate solvent fungus test on Pikovskaya media. The results of the identification of the fungi obtained as follow: Aspergillus niger, Aspergillus bertholletius, Aspergillus flavus, Aspergillus isolate 4, Aspergillus isolate 5, Penicillium citrinum, and Trichoderma amazonicum. From the entire types of fungi obtained, there are onlybfour fungi that have the potential as phosphate solvents, namely Aspergillus niger, Aspergillus flavus, Aspergillus bertholletius and Penicillium citrinum with the presence of clear zones on Pikovskaya media. Fungi that has the best potential in the process of phosphate dissolution is Aspergillus niger.
 
 Key words: Rhizosfer, Lantana camara, clear zone, phosphate solvent fungus
Highlights
identify microscopic fungi to be used as phosphate solvent fungi
which later will be taken from each plant soil samples
that the presence is very common in Taman Nasional Bali Barat (TNBB)
Summary
Pengambilan sampel tanah dilakukan dengan cara menggali tanah rhizosfer dengan kedalaman 20 cm di setiap zona TNBB sebanyak lima titik dari tiap zona sehingga diperoleh sampel sebanyak 25. Isolasi Sampel Tanah Metode yang dilakukan pada sampel tanah yaitu metode pengenceran 10-1-10-4. Sampel ditimbang sebanyak 10 gr, kemudian dimasukkan ke dalam botol yang berisi air steril 90 mL dan dikocok hingga homogen. Selanjutnya diambil 1 mL dari setiap tabung reaksi untuk dimasukkan ke dalam cawan petri untuk dituangkan media PDA, dan dihomogenkan. Biakan murni dibuat dengan mengambil spora jamur pada sampel yang telah diisolasi menggunakan jarum ose dan dibiakkan pada cawan Petri yang telah diisi media PDA. Secara makroskopis variabel yang diamati adalah warna koloni, warna sebalik koloni, ada atau tidaknya garis radier atau konsentrispermukaaan koloni, pengamatan secara mikroskopis dilakukan dengan cara melihat hifa atau spora pada obyek glass menggunakan mikroskop. Hasil yang diperoleh selanjutnya disesuaikan dengan buku kunci identifikasi jamur (Proborini, 2011)
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