Abstract
1. 1. Phosphomono- and phosphodiesterases from several microorganisms and two photosynthetic tissues have been examined by gel electrophoresis. Each species has a unique pattern of these two enzymes. 2. 2. A nondestructive method for locating these enzymes in polyacrylamide gels is described that enables one to differentiate between phosphomono- and phosphodiesterases and that provides a rapid method for identifying cell extracts from the patterns of phosphatases present. 3. 3. Substrate affinity chromatography on cellulose phosphate columns has been successfully used to separate phosphomonoesterases from crude cell extracts. Also columns of glyceryl cellulose phosphate retard the elution of phosphodiesterases but not other proteins. 4. 4. This affinity chromatography can be used to remove phosphatases from crude extracts or to purify phosphatases, either phosphomono- or diesterases.
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