Abstract
Phenotyping hemoglobin variants of a flock of adult Altamurana sheep led to individuals with an unusual level of polymorphism being found. To find the best hemoglobin band resolution, isoelectric focusing (IEF) in traditional and in immobilized pH gradients (IPGs) of six different hemoglobin phenotypes were compared. Such a comparison afforded the following results: (1) all hemoglobin variants focus in a range of 0.7 pH units (6.7–7.4); (2) traditional IEF in the presence of chemical spacers allows the resolution of α 8Ala hemoglobin which usually remains undetected; (3) owing to the high number of hemoglobin variants in the samples, IPGs were performed in a range of 1 pH unit and exhibited almost the same results as IEF in the presence of chemical spacers. In conclusion with traditional IEF procedures, chemical spacers provided improved resolution of hemoglobin variants and the same results as IPG gels in 1 pH unit.
Published Version
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