Phenotypic detection of ESBL among E.coli and Klebiella pneumoniae by modified CLSI guidelines

Abstract

Introduction: Escherichia coli and Klebsiella pneumoniae cause a wide range of infections. Multidrug-resistance strains carrying resistance genes have become a growing problem worldwide. The ESBLs have emerged distinctly, especially in Escherichia coli and Klebsiella pneumoniae. Material and Methods: 250 non repetitive isolates of E. coli and K. pneumoniae were isolated from different clinical samples (pus, urine, sputum, blood) for the study. Each isolate was tested for production of ESBL by CLSI recommended PCT and compared with Modified CLSI Method for ESBL detection. Results: 133 E. coli and 117 K. pneumoniae were isolated where 65.2% of ESBL were detected. With modified CLSI method, overall rate of ESBL positivity increased from 65.2% to 74.4%.of E. coli and 74.3% of K. pneumoniae isolates were ESBL producers by modified CSLI Method. Conclusion: All the clinical samples growing gram negative bacteria should be tested for ESBL, production. Considering the grave scenario of antibiotic resistance in our country, it is high time that all clinical laboratories start detecting these enzymes routinely and accurately. Keywords: Escherichia coli, Klebsiella pneumoniae, ESBL, Modified CLSI guidelines.