Phenotypic and Molecular Characterization of Pseudomonas aeruginosa Isolated from Human specimens

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is considered an opportunistic pathogen that causes high morbidity and mortality and is responsible for a high proportion of hospital infections pathogen that can cause several disease syndromes in the community and hospitals. Recently, P. aeruginosa infections having antibiotic resistance have been increasing and serious problems are being experienced in treatment. This study aimed to determine the prevalence, antibiogram pattern, and molecular identification of oprL virulence gene of P. aeruginosa recovered from human pus. One hundred samples from human pus were collected for bacteriological identification before being subjected to molecular identification. The results revealed that 13/100 (13%) were positive for P. aeruginosa on Citramide ager. All isolates showed high sensitivity to Imipenem, Apramycin, Amikacin, Norfloxacin, Sulphamethoxazol, with 100%, 92.31%, 84.61%, 9.23%, and 61.53% respectively. For the molecular characterization of P. aeruginosa (5 isolates) for detection of oprL virulence gene. The results indicated that oprL genes were the detected genes in all tested samples with (100%).In conclusion; the obtained results provide important data about the importance of the oprL virulence gene and pathogenicity of P. aeruginosa as well as the widespread of MDR strains that complicated the treatment options in human community