Abstract
Back ground: S. aureus especially methicillin and multi drug resistant S. aureus (MRSA and MDRSA) has the tendency to form biofilm. Our aim was to study the in vitro biofilm-forming ability of S. aureus isolates and to examine the relationship between biofilm formation and antibiotic resistance pattern. Methods: One hundred and forty one clinical isolates of S. aureus were isolated from wound pus and tracheal aspirate samples. Biofilm formation of these isolates was detected by Congo red (CRA) method and micro-titer plate (MTP) methods and confirmed by PCR through detection of biofilm genes. Results: The percentage of biofilm-producing isolates was found to be 81.6% and 58.2% for MTP and CRA methods respectively. The majority of MRSAisolates were positive for biofilm genes. Conclusions: Biofilm-producing isolates exhibit high tendency to develop multidrug resistance and methicillin resistance.
Highlights
The concept of biofilm was previously defined by Fletcher and Floodgate (1973), “Biofilm is the unique pattern of growth in the life cycle of microbes that provides specific properties, advantages and higher level of organization of free living bacterial cells during colonization” [1]
Bacterial isolates Out of 290 bacterial isolates, 141/290 (48.6%) isolates were phenotypically identified as S. aureus, 94(66.7%) isolates were from burn or surgical wound samples of the plastic surgery department and 47(33.3%) isolates were from tracheal aspirate samples of intensive care unit (ICU) Neurological Department
All isolates of S. aureus (141) were tested for their ability to form biofilm using Congo red agar (CRA) and micro-titer plate (MTP) methods are shown in supplementary figure (1& 2)
Summary
The concept of biofilm was previously defined by Fletcher and Floodgate (1973), “Biofilm is the unique pattern of growth in the life cycle of microbes that provides specific properties, advantages and higher level of organization of free living bacterial cells during colonization” [1]. The formation and development of biofilms is a complicated procedure including, different stages. Staphylococcus aureus (S. aureus) especially methicillin and multi drug resistant S. aureus (MRSA and MDRSA) has the tendency to form biofilm. Our aim was to study the in vitro biofilm-forming ability of S. aureus isolates and to examine the relationship between biofilm formation and antibiotic resistance pattern. Methods: One hundred and forty one clinical isolates of S. aureus were isolated from wound pus and tracheal aspirate samples Biofilm formation of these isolates was detected by Congo red (CRA) method and micro-titer plate (MTP) methods and confirmed by polymerase chain reaction (PCR) through detection of biofilm genes. Results: The percentage of biofilm-producing isolates was found to be 81.6% and 58.2% for MTP and CRA methods respectively. Conclusions: Biofilm-producing isolates exhibit high tendency to develop multidrug resistance and methicillin resistance
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