Abstract
This work was planned to investigate the prevalence of P. aeruginosa in broiler chickens kidneys suffered from naked eye pathological lesions. Out of 240 examined kidneys samples, 25 isolates (10.4%) of P. aeruginosa were recovered. Antimicrobial sensitivity pattern against 14 different antimicrobials comprising nine groups proved that isolates were resistant to most of the used antimicrobial agents. Phenotypic detection of extended spectrum beta-lactamases (ESBLs) producing isolates using modified double disk diffusion test revealed that, ten (40%) were ESBLs producers. Genotypically using PCR, three ESBLs genes (TEM, Ctx-M and Shv) and three virulence genes (lasI, Tox A and Plc H) were detected with different percentages. Cinnamon and carvacrol oils were found bactericidal for P. aeruginosa at concentrations of 4% and 0.4% respectively.
Highlights
Bacterial lesions in kidneys usually developed when bacteria enter the kidney following systemic disease through the renal arteries or the renal portal system or as a result of ascending infection (Schmidt et al, 2015)
Results of phenotypic detection of extended spectrum beta-lactamases (ESBLs) sprodusing isolates: All 25 P. aeruginosa isolates under study showed either resistance or intermediate susceptibility pattern to one or more of the extended spectrum betalactamases were subjected to modified double disk diffusion test to confirm ESBLs production
ESBLs were confirmed phenotypically in 10 (40%) out of 25 isolates of P. aeruginosa recovered from inflamed kidneys
Summary
Bacterial lesions in kidneys usually developed when bacteria enter the kidney following systemic disease through the renal arteries or the renal portal system or as a result of ascending infection (Schmidt et al, 2015). Pseudomonas aeruginosa is a motile, Gram-negative, oxidase positive, non-spore forming rods with single arrangement or short chains (Elsayed et al, 2016). It producesa water soluble green pigment composed of fluorescentand pyocyanin with a specific fruity odor. P.aeruginosa considered being an opportunistic pathogen that can cause respiratory infections, septicemia and other forms when affecting birds (Fekadu, 2010). It affects newly hatched chickens drastically causing high mortality and mass death of aeruginosa to various antimicrobial agents is contribute to impermeability, multi-drug efflux, and a chromosomal AmpC β-lactamase.
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