Abstract

Preimplantation embryo culture is a valuable approach to investigate a preimplantation lethal phenotype. Standard culture methods have been perfected such that the entire preimplantation period and the process of implantation can be followed in vitro. This protocol provides modifications for the analysis of clutches of embryos from heterozygous matings specifically for the purpose of distinguishing a preimplantation phenotype in homozygous mutants.

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