Phenotype and function of lymphocytes in normal and inflamed appendix

Abstract

Event Abstract Back to Event Phenotype and function of lymphocytes in normal and inflamed appendix Ghasem Mosayebi1* 1 Arak University of Medical Sciences, Immunology, Iran Introduction: The appendix is considered as part of the gut- associated lymphoid tissue. The function of appendix is not clearly defined. In this study to understand better the immunological function of the appendix, we investigated the function and phenotypic pattern of lymphocyte subsets in peripheral blood and appendix of patients with normal and inflamed appendix tissue. Patients and Methods: Peripheral blood samples and appendiceal mononuclear cells obtained from 80 patients (mean age; 23±10.5), who were clinically suspected of having appendicitis. Based on histopathological examination, twenty-five patients had normal appendix while 40 and 16 were diagnosed with acute and gangrenous/perforate appendicitis, respectively. The phenotypic characteristics of lymphocyte subsets in blood and tissue analyzed by three color-flow cytometry. The proliferative responses of peripheral blood or tissue mononuclear cells assayed by MTT method. Results: The frequency of CD19/DR, HLA-DR and CD19+-cells in the appendix tissue were significantly higher than the peripheral blood in all the groups (p<0.001). Overall, there were significantly higher percentage of CD5, CD3, CD8, α/β-TCR, RA/RO, CD3/RA in peripheral blood of normal patients than were found in appendix mononuclear cells (p<0.01). In patient with acute appendicitis, the percentage of CD5, CD3, CD8, α/β-TCR, CD3/CD38 and CD3/RA were significantly different between blood and tissue (p<0.001). There were significantly different in percentage of CD19, HLA-DR and CD3/CD4/αβ-TCR between acute, gangrenous appendicitis patients and patients with normal appendix. There is significant different in the proliferative responses of the peripheral blood and appendiceal mononuclear cells to PHA and Con-A between acute, gangrenous appendicitis patients and patients with normal appendix (p<0.01). Conclusion: The phenotypic and function of lymphocytes are different between peripheral blood and appendix tissue. Also, lymphocytes phenotypic changes in peripheral blood of appendicitis patients can be used as a diagnostic marker for appendicitis. Keywords: Appendix, Appendicitis, phenotype, Lymphocytes, proliferation, Flow Cytometry Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Mosayebi G (2013). Phenotype and function of lymphocytes in normal and inflamed appendix. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00661 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Ghasem Mosayebi, Arak University of Medical Sciences, Immunology, Arak, Iran, gmosayebi@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ghasem Mosayebi Google Ghasem Mosayebi Google Scholar Ghasem Mosayebi PubMed Ghasem Mosayebi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.