Abstract

The present study aimed to estimate the individual and total phenols and antioxidant activity of the aqueous and alcoholic extracts of three premium quality date varieties (Khalas, Sukkari and Ajwa) from Saudi Arabia. In general, water extract has shown significantly higher contents of total phenols than alcoholic, especially in Ajwa (455.88 and 245.66 mg/100 g respectively). However, phenolic profile indicated that Sukkari contained the highest rutin concentration (8.10 mg/kg), whereas, catechin was approximately the same in Sukkari and Ajwa (7.50 and 7.30 mg/kg respectively). Khalas was the highest variety content of caffeic acid (7.40 mg/kg). A significant difference has indicated among extracts and varieties in suppressing lipid peroxidation. Sukkari and Ajwa have reduced the oxidation with 50% at lower concentration in water extract than alcoholic (0.63, 0.70 and 1.60, 1.43 mg/ml respectively). Furthermore, high positive linear correlation was found between total phenols in water (r = 0.96) and alcohol (r = 0.85) extracts and inhibition of lipid oxidation activity. The compounds responsible for the activity were catechin (r = 0.96), and rutin (r = 0.74) in water extract, whereas this correlation decreased in alcoholic extract (r = 0.66) for catechin and very weak (r = 0.38) for rutin. No correlation was found between caffeic acid and lipid peroxidation in both extracts. Similar significant results were obtained with DPPH test, except with Sukari, which has shown no difference between aqueous and alcoholic extracts (4.30, 4.10 mg/ml respectively).

Highlights

  • Fruits and vegetables have been implicated in preventing or reducing the risk of coronary heart diseases [1,2], cancer [3,4] and other chronic diseases [5]

  • The compounds responsible for the activity were catechin (r = 0.96), and rutin (r = 0.74) in water extract, whereas this correlation decreased in alcoholic extract (r = 0.66) for catechin and very weak (r = 0.38) for rutin

  • Phoenix dactylifera L., is an important plantation crop for many countries extending from North Africa to the Middle East including many states of the Arabian Gulf Cooperation Countries (GCC)

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Summary

Introduction

Fruits and vegetables have been implicated in preventing or reducing the risk of coronary heart diseases [1,2], cancer [3,4] and other chronic diseases [5]. It was found that palm date has a potent ability to suppress free radicals using different methods [13,14] even within studies that found little contents of total phenols [11,12]. Phenolic Contents and Antioxidant Activity of Various Date Palm (Phoenix dactylifera L.) Fruits from Saudi Arabia 1135. The total phenolic compounds (TPC) and antiradical efficiency of the Algerian ripe date palm fruits were 2.49 - 8.36 mg gallic acid equivalents (GAE) per 100 g fresh weight and 0.08 - 0.22 mg/100g, respectively. The aims of this study were to estimate the individual and total phenols in three date varieties, considered to be premium quality and the most consumed in the Kingdom of Saudi Arabia namely Khalas, Sukkari, and Ajwa, their antioxidant activity, and determine the correlation between the antioxidant capacity and phenolic contents

Plant Materials
Extraction of the Phenolics
Flavonoids and Phenolic Compound Quantification
Free Radical Scavenging Capacity
Total Phenols and Glycosides in Dates
Flavonoides and Phenolic Compounds
Antioxidant Activities
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