Abstract
Phenolic compounds are functional food nutrients and show structural similarity, synthesized through phenylpropanoid and flavonoid biosynthetic pathway. Their structural similarity makes the separation and structure elucidation challenging. In this study, we isolated and identified phenolic compounds in tomato fruit by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Total 32 phenolic compounds were found and 13 out of them were previously unknown in tomato fruits. We elucidated nine from the 13 previously unknown components. The 3 main phenolic compounds were the same in both selected wild and cultivated tomato fruits, including chlorogenic acid, rutin and naringenin. Nevertheless, the content difference of the 3 main and 10 previously unknown phenolic compounds caused significant higher phenolic level in wild tomato fruits, compared to cultivated tomato. Based on the identified phenolic compounds, we also study their content variation in phenylpropanoid and flavonoid pathway of tomato fruit. Our results are beneficial for the improvement of phenolic nutrients in tomato fruits.
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