Abstract
In oxygen-limited continuous culture,Saccharomyces cerevisiaeformed pseudohyphae by unipolar budding. We developed a continuous cultivation sequence to discriminate phase-specific from metabolically regulated proteins during dimorphism. Computer-aided substractive analysis of 2D–PAGE protein patterns allowed the detection of proteins specifically expressed during yeast and pseudohyphal phases. Image analysis resolved 3 spots that were specific to the pseudohyphal phase and 2 spots that were specific to yeast phase. In addition to phase-specific proteins, important regulation of protein expression took place. A group of 9 proteins was highly over-expressed during the yeast phase when another group of 12 was under-expressed. This phenomenon was reversed during the pseudohyphal phase. These experiments showed that dimorphism inS. cerevisiaeis associated with the expression of specific proteins and suggest that yeast phase-specific proteins maintain the yeast form or repress pseudohyphae formation.
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