Abstract
Recent literature has shown that buffers affect the interaction between lipid bilayers through a mechanism that involves van der Waals forces, electrostatics, hydration forces and membrane bending rigidity. We endeavour to show phase coexistence as an effect of charges from the aqueous boundary layer on the mixed chain 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid bilayers. We will present data that suggests one phase dehydrates below the value in pure water while the other phase swells as the concentration of buffer is increased. However, since the two phases must be in osmotic equilibrium with one another, this behavior challenges theoretical models of lipid interactions and introduces new variables to consider for the Gibbs phase rule. This model of lipid charging was then applied to explain the mechanisms behind phase separation in lipid mixtures containing phosphatidylinositols.This work was supported by NIH NCI K01-CA169078-01 and Indiana University Collaborative Research Grants.
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