Pharmacological, histopathological, and biochemical assessments of cutaneous wound healing potential of Tragopogon graminifolius aqueous extract ointment in rats

Abstract

The recent experiments have revealed the property of ethno-medicinal plants on the treatment of the cutaneous wound. The purpose of the experiment was to investigate the wound healing potential of Tragopogon graminifolius aqueous extract ointment. DPPH free radical scavenging test was carried out to examine the antioxidant effect of T. graminifolius aqueous extract, which indicated similar antioxidant activity with butylated hydroxy toluene (BHT) as a positive control. In vivo design, 120 Sprague Dawley male rats were used. After creating the cutaneous wound, the animals were randomly divided into four groups; untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% T. graminifolius aqueous extract ointment (3 g of T. graminifolius aqueous extract + 97 g base ointment). On days 10, 20, and 30 after creating the wound, for histopathological and biochemical analysis of the cutaneous wound healing trend, a section was prepared from all dermal thicknesses. The data were analyzed using one-way ANOVA followed by Duncan post hoc test. On days 10, 20, and 30, T. graminifolius aqueous extract ointment could significantly (p ≤ 0.05) decrease the level of the wound area, total cell, macrophage, lymphocyte, and neutrophil, and enhance the level of wound contracture, fibrocyte, hexuronic acid, hexosamine, and hydroxyproline as compared to the basal ointment and control groups. In conclusion, the acquired findings showed the cutaneous wound healing potential of T. graminifolius aqueous extract ointment.