Antioxidant, cytotoxic, and wound healing effects of Allium saralicum R.M. Fritsch aqueous extract ointment in male rats: a gross, histological, and biochemical experiment

Abstract

In recent years, cutaneous wound healing by medicinal plants has been on the raise. In recent research, we appraised antioxidant, cytotoxicity, and cutaneous wound healing activities of Allium saralicum R.M. Fritsch aqueous extract ointment in rats. In this study, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was carried out to test the antioxidant property, which revealed high antioxidant potential similar to butylated hydroxytoluene (BHT) as a positive control. Synthesizing the aqueous extract dose-dependently demonstrated great cell viability and that this method was non-toxic for synthesizing A. saralicum. In vivo experiment, after creating the cutaneous wound, the animals were randomly divided into four groups: untreated control, treatment with Eucerin basal ointment, treatment with 3% tetracycline ointment, treatment with 10% A. saralicum ointment. These groups were treated for 30 days. For biochemical and histopathological analysis of the healing trend, a 3 × 3-cm section was prepared from all dermal thicknesses on days 10, 20, and 30. The use of A. saralicum ointment in the treatment groups led to substantial diminish (p < 0.05) in the levels of wound area, total cells, neutrophil, macrophage, and lymphocyte, and remarkable raise (p < 0.05) in the levels of wound contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and the rate of fibrocyte to fibroblast as compared with the control and basal ointment groups. Adapting to the findings, A. saralicum ointment can cure the cutaneous wound without any cytotoxicity.