Abstract
<p class="Abstract">The present study is aimed to investigate the phytochemical screening and biological activities of methanolic extract of <em>Cyperus scariosus</em> roots. Dried plant was grounded and extracted with methanol to prepare methanol crud extract. In vitro biological tests were conducted using this methanolic extracts according to the standard procedure. 100% death rate of brine shrimp was perceived at 3 mg/mL of plant extract after 72 hours. The extract showed action against <em>Aspergillus flavius</em> i.e. 90% followed by A. niger (91%) while the highest activity was shown against <em>A. fumegatrus</em> (94%). Important scavenging results were detected during scavenging of free radicals viz; 92.2% against DPPH, 82.2% to ABTS, 75.8% to hydrogen peroxide, 88.1% to β-carotene, 86.1% to hydroxyl radical and 89.4% against phosphomolybdate at 3 mg/mL were obtained. The results obtained in this study point out that extract showed significant biological activities which might be due to the presence of bioactive constituents.</p><p> </p>
Highlights
In the previous decade, there has been a rising interest in the medical consequences of free radicals
The extract showed activity against A. flavius i.e. 90% followed by A. niger (91%) while the highest activity was shown against A. fumegatrus (94%)
The ABTS (2,2, azo-bis-(3-ethyl benzothiazoline-6sulphonic acid) free radical scavenging capacity of the sample extract was less than the standard ascorbic acid (Table II)
Summary
There has been a rising interest in the medical consequences of free radicals. With certain modifications the ABTS radical scavenging assay was measured using the method developed by Re et al (1999). Free radical scavenging activity was determined by mixing 100 μL of different concentrations (3 mg/mL, 1.5 mg/mL, 0.75 mg/mL and 0.37 mg/mL in methanol) with 1 mL of ABTS working standard. Hydroxyl radical scavenging activity of extracts was examined by the method of Halliwell and Gutteridge, (1981).
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