Pharmacological Correction of the Results of Irradiation of the Parents for the Assimilation of Carbohydrates in the Small Intestine of Two Generations of their Offspring by the Help of Milk Thistle Fruits

Abstract

Objective: The study of the assimilation of glucose and maltose in the small intestine of two generations of posterity of irradiated male rats received with a food the milled milk thistle fruits after irradiation. Methods: Exposition of male rats performed once by the help of telegamma-setting “AGAT-R1”, dose was 120 rad/min, field 20×20, distance from the source of irradiation to the field=75 cm, dose-0.5 Gy, exposure - 32 sec. Accumulating mucosa preparations (APS) produced by the method of Ugolev et al. APS incubated for 1 hour at t=37oC in the oxygenated medium. As the incubation medium were used solutions 10 mmol/l of glucose and 5 mmol/l of maltose that were made on the Ringer solution pH 7.4. In all incubation medium the bile was added. The concentration of free glucose and M-glucose formed due to the hydrolysis of maltose was determined using method described in ref. Colorimetrically on photoelectrocolorimeter–CFC- 2MP, λ=625 nm. The statistical processing of the obtained data was conducted using “Primer Biostatistics” software. Results: The 2nd generation of the offspring from the males, which received milk thistle in the food immediately after irradiation, showed the high activity of the transport system for the free glucose and system providing transport of M-glucose produced by the hydrolysis of maltose both. This activity is higher than in the corresponding groups of the 1st generation (p<0,0001) and higher than corresponding data in all experimental groups of 2nd generation (except only group of the posterity of the male rats got milk thistle fruits before irradiation). Conclusion: The use by predecessors of milk thistle after irradiation leads to the radio corrective effect on the systems of transport of glucose and maltose both exactly in second generation of their posterity.