Pharmacological activity of sea cucumber Cucumaria frontosa derived amylase enzyme against blood stream pathogen

Abstract

At presently availability of resistance pathogen to numerous drugs is a common feature of hospital-acquired Pseudomonas aeruginosa strains. So, the present study collect the 80 blood sample from blood cancer patient in Revathi Medical Hospital, Tirupur City for isolating the PA strain from blood sample and confirmed the bacterial strain using standard test method followed by drug susceptibility test for identification of resistant’s. In this study the maximum resistant was recorded in antibiotics Vancomycin 99% and maximum sensitive was observed in antibiotic Levofloxacin 99%. The blood stream infection causing PA plasmid extracted in 19 carcinogenic isolates which was showed more than 50% resistance selected for plasmid isolation. Due to the ability of resistant mechanism present in BSI pathogen the identification of new resources for novel antibiotics used urgently need to carry out a specific task to deal the blood stream infection. Moreover the enzyme especially the amylase have been isolated and purified from marine sea cucumber associated microorganism and the antimicrobial assay was done by using well techniques. The maximum zone of inhibition at 50, 100 and 150µl (, 25mm, 26mm and 16mm, 22mm, 23mm) of amylase extract was observed in the PAVG43,60 isolates. So hence this motto of this research proved the sea cucumber associated extracted amylase enzyme play a major role in the diagnosis of blood stream infection in cancer patients.
 Keywords: Blood Stream Infection, Pseudomonas aeruginosa, Sea Cucumber, Amylase