Pharmacokinetic Comparisons of Eight Active Components from Raw Farfarae Flos and Honey-Processed Farfarae Flos after Oral Administration in Rats by UHPLC-MS/MS Approaches.

Liu Yang,Jianmin Li,Bingyou Yang,Ajiao Hou,Wenjing Man,Song Wang,Xinyue Guo,Jiaxu Zhang,Haixue Kuang,Hai Jiang

doi:10.1155/2020/4091816

Abstract

Farfarae flos (FF) is widely used for cough over thousands of years in China, but little is known about their pharmacokinetics properties. This study was aimed to establish a rapid and accurate ultraperformance liquid chromatography with triple-quadrupole tandem mass spectrometry method for compare pharmacokinetics studies of eight active compounds after oral administration between raw and honey-processed farfarae flos extracts. Optimum separation was performed on a Thermo Hypersil GOLD C18 column (100 mm × 2.1 mm, 1.9 µm particles size) with a gradient elution of acetonitrile as mobile phase A and 0.3% formic acid aqueous solution as mobile phase B. The flow rate was set as 0.3 mL/min and separated for 34.0 minutes. Electrospray ionization in the negative ion mode and selected reaction monitoring were used to identify and separate active components. The results met the acceptance criteria and showed that this method exhibited good linear, precision, accuracy, and stability. The extraction recoveries ranged from 81.54% to 104.48%, and the matrix effects ranged from 81.94% to 103.02%. These results show that the validated method could be successfully applied to evaluate the pharmacokinetic study in rats after oral administration of raw farfarae flos (R-FF) and honey-processed farfarae flos (H-FF).

Highlights

Before traditional Chinese medicine (TCM) is used for their specified clinical application, its raw herbs are commonly specially processed, which is called “paozhi” in Chinese [1]
Even though Farfarae flos (FF) have a long history of clinical application, little is known about their pharmacokinetics properties. us, the dynamics of raw and honey-processed farfarae flos (H-FF) in vivo are not well understood on account of absence of scientific evidence and research method
We first chose the ACQUITY UPLC 1.8 μm HSS T3 column (Waters) to carry out elution, but we found partial peak trailing. en, we chose 1.9 μm Hypersil GOLD column to carry out elution, and we found that all compounds could rapidly and complete separation

Summary

Introduction

Before traditional Chinese medicine (TCM) is used for their specified clinical application, its raw herbs are commonly specially processed, which is called “paozhi” in Chinese [1]. Traditional Chinese medicine processing methods are wine-processed, salt-processed, vinegar-processed, honey-processed, Glycyrrhizae Radix et Rhizomaprocessed, sulfur fumigation, and so on [2, 3]. E purpose of TCM processing is to promote therapeutic effects, eliminate toxicity, reduce side effects, and change the properties of raw herbs. It is important that H-FF is the best choice of cough medicine, which has a long history been widely applied in many Chinese patent medicines such as Zhike Juhong Koufuye and Zhisou Qingguo Heji [7, 8]. Even though FF have a long history of clinical application, little is known about their pharmacokinetics properties. Even though FF have a long history of clinical application, little is known about their pharmacokinetics properties. us, the dynamics of raw and H-FF in vivo are not well understood on account of absence of scientific evidence and research method

Methods

Results

Conclusion