PHARMACOGNOSTICAL, PHYTOCHEMICAL AND ANTIBACTERIAL EVALUATION OF MAHONIA LESCHENAULTIA TAKEDA. (ROOT WOOD & ROOT BARK).

Abstract

Objective: Pharmacognostical, Phytochemical and Antibacterial evaluation of Mahonia leschenaultii Takeda. (root wood, root bark, stem wood, stem bark and leaves.) Methods: The present study has been undertaken to evolve the Pharmacognostical, Phytochemical and Antibacterial standard. In Pharmacognostical study determination of ash values and extractive values were carried out. In Phytochemical study Extraction procedure and Fluorescence analysis were carried out. In Antibacterial study the antibacterial activity of different methanolic extracts (root wood, root bark, stem wood, stem bark and leaves extracts.) of different concentration (50 µg/ml, 100 µg/ml, 150 µg/ml and 200 µg/ml) and standard drug (Ampicillin trihydrate) at the concentration of 100 µg/ml were carried out by cylinder-plate method. Results and Discussion: Pharmacognostical studies such as ash values and extractive values were carried out to confirm the identity of plant and to ascertain the quality and purity of crude drug. Phytochemical study such as Fluorescence analysis shows fluorescence compound in the extracts. The antibacterial study shows the moderate antibacterial activity against Staphylococcus aureus and Escherichia coli in comparison to standard drug (Ampicillin trihydrate). Conclusion: Pharmacognostical studies were carried out to confirm the identity and to ascertain the quality and purity of the crude drug. Phytochemical studies were carried out to confirm the presence, nature of the crude extracts. The extracts of (root wood, root bark, stem wood, stem bark and leaves) of different concentration (50 µg/ml, 100 µg/ml, 150 µg/ml and 200 µg/ml) evaluated for antibacterial activity and showed moderate antibacterial activity as compared to standard drug at the concentration of 100 µg/ml. The methanolic extract is more active against Escherichia coli in comparison to Staphylococcus aureus. Keywords: Mahonia leschenaultii Takeda. Root wood, Root bark, Stem wood, Stem bark and leaves, Ampicillin trihydrate.Â