Abstract
Aims: The purpose is to study Pharmacognostical characteristics, Physicochemical evaluation, Phytochemical study and Antimicrobial assay of Curcuma longa leaves extract. Methods and Material: Different qualitative tests were performed to confirmed the presence of phytochemical compounds under consideration followed by quantitative analysis using sophisticated techniques such as spectrophotometric, Thin layer chromatography (TLC) and column chromatography. Results: In pharmacognostic study the moisture content of Curcuma longa leaves is 1.75%, water and alcohol soluble extractive value is 19.25 and 12.6 % respectively, total ash obtain is 3.35%. Acid insoluble and water-soluble ash value is 1 and 1.2% respectively. phytochemical analysis of Curcuma longa was carried out by using various solvent (methanol, acetone and aqueous) extract crude sample for presence of secondary metabolites. The qualitative phytochemical analysis showed the presence of cumarin, fatty acids, terpenoids, tannin, phenolic compounds, flavonoids, reducing sugar and glycosides in respective solvent extract. The quantitative estimation of total phenolic content of Curcuma longa leaf extract for methanol, acetone and aqueous are (1.52,1.22 and 0.46). and for the flavonoid content of Curcuma longa leaf extract for methanol, acetone and aqueous are (1.46,1.34 and 0.78). the antimicrobial activity of Curcuma longa leaves extract against human pathogenic microorganisms (E. coli and B. thurengenesis). And one species of fungus (A. niger) by paper diffusion assay method performed on nutrient agar and Potato dextrose agar respectively. In antimicrobial analysis methanol and acetone extract was effective against E. coli and B. thurengenesis. The inhibitory zone was found to be ranging from 8mm to 20mm in diameter. Conclusions: This study may be useful a source of information concerned to various field such as pharmacology, ethnology, phytochemistry and industrial microbiology for future aspect. KEYWORDS: Quercetin, Curcuma longa leaves extract, Thin layer chromatography, Column chromatography, Spectrophotometry, antimicrobial assay, Phytochemical screening, Pharmacognostic study
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