Abstract
Aim: To rationalize the macroscopial, microscopical and physico-chemical studies on leaves of plant Nyctanthes arbor-tristis Linn. Methods: The pharmacognostic characters were determined in terms of macroscopy, microscopy and powder microscopy of plant Nyctanthes arbor-tristis leaves. The crude ethanolic extract of leaves of Nyctanthes arbor-tristis Linn. was using physico-chemical parameters, and preliminary phytochemical investigation (TLC). Results: The microscopic study shows the general characteristic of Nyctanthes arbor-tristis leaves. Phytochemical analysis revealed the presence of various phytochemical groups like alkaloids, glycosides, steroids, phenolic, tannins constituents. Conclusion: It can be concluded that the established pharmacognostic profile of Nyctanthes arbor-tristis leaves will be helpful in developing pharmacopoeial standards for correct identification and quality control. The present observation will also be helpful in macroscopical and microscopical on leaves of Nyctanthes arbor-tristis Linn.
 Keywords: Nyctanthes arbor-tristis, Pharmacognostical Study, Macroscopial and Microscopical studies
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