Abstract
Objective: To evaluate the diagnostic pharmacognostical characters of Costus spicatus in rhizomes extract along with their physico-chemical parameters and fluorescence analysis.
 Methods: The pharmacognostical characters were determined in terms of macroscopy, microscopy, Powder microscopy, rhizome constant, fluorescence analysis and Preliminary phytochemical investigation.
 Result: The fluorescence characteristics of rhizome powdered extracts were determined under UV radiation of long and short wavelengths and ordinary visible light. When the powdered drug and extracts were treated with different reagents and observed under UV and ordinary light, they emitted various colour radiations. The colour change for the rhizome powder and individual extract were distinctive and reproducible revealing the solvent properties to the phytoconstituents and data is present. The moister content was calculated through the loss on drying method and was found to be 1.43%. The swelling and foaming index values were recorded. The phytochemical investigation indicates the presence of the organic phytoconstituents such as alkaloids, carbohydrate, flavonoids, protein, amino acids, phenols, tannin, glycosides, steroids and inorganic constituents like iron, phosphate, sulphate and chlorides.
 Conclusion: Various pharmacognostical and physicochemical parameters have pivotal roles in identification, authentication and establishment of quality parameters of the species.
 Keywords: Costus spicatus, Physico-Chemical parameters and fluorescence analysis.
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