Pharmacognostic Evaluation and Quantification of Quercetin in Muntingia Calabura Leaves and Fruits Using HPLC Method

Abstract

The present is to evaluate the pharmacognostic character of Muntingia Calabura and estimation of quercetin in flavonoid rich leaves and fruit fractions. Pharmacognostic studies were carried out in terms of morphological, microscopic characters, physicochemical parameters and HPLC fingerprint analysis of leaf and fruit extracts of Muntingia calabura samples using standard methods. Further, the extracts were used to analyze total phenol, flavonoid contents and estimated antioxidant activities using DPPH. fruit size. and color of Muntingia calabura was the distinguishing morphological character observed in the present study. The detailed powder microscopy of leaf confirmed the presence of fibers, trichomes, vascular bundle and Xylem vessel. and fruits with specified Epicarp, Reticulate xylem vessel, starch grains, Endosperm, oil globules, parenchymatous tissue. Physicochemical parameters like ash values, and extractive values. were determined. Preliminary phytochemical screening showed the presence of alkaloids, glycosides, steroids, carbohydrates and flavonoids. Using HPLC Profiling, Standard quercetin was found to have a retention time of 7.313 while flavonoid-rich leaf and fruit had retention times of 7.245 and 7.210, respectively. Based on the results of the research, it was discovered that Muntingia calabura, fruit and leaves contain a variety of secondary metabolites with strong antioxidant ability. The morphological and histological traits of Muntingia calabura, leaves and fruit can be witnessed under a microscope. Using HPLC Profiling for Quantification of Quercetin in flavonoid rich fraction of leaves and fruits.