Abstract
Phage display technology is a unique gene recombination expression technology, and it is also a simple and effective screening tool. Through panning, a protein or peptide with high affinity and selectivity to the target is obtained. Antibody phage display has become the first and most widely used in vitro screening technology. Phage display derivatives play an important role in the diagnosis and treatment of diseases. This article reviews the phage display system of phage display technology, the size and classification of antibody libraries and their applications, and discusses the application prospects and challenges of phage display technology. This thesis lays the foundation for the theoretical and experimental research of bacteriophages.
Highlights
Phage display derivatives play an important role in the diagnosis and treatment of diseases
Phage display technology was first proposed by Smith in 1985
The final round of panning phage monoclonal antibodies is screened and the selected monoclonal antibodies are obtained by DNA sequencing
Summary
Phage display technology was first proposed by Smith in 1985. Smith successfully integrated exogenous DNA into M13 phage, and expressed the exogenous polypeptide with the coat protein of M13 phage. McCafferty et al described antibody phage display for the first time They were able to fuse the encoding scFv to gene III. Without affecting the infectivity of the phage and maintaining the functionality of the antibody, the scFv was fused with the capsid protein and expressed and displayed on the surface of the phage. This method is different from other bacterial expression systems in that the protein (phenotype) is directly linked to its homologous gene (genotype) through phage. The final round of panning phage monoclonal antibodies is screened and the selected monoclonal antibodies are obtained by DNA sequencing
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