PH affects the hormesis profiles of personal care product components on luminescence of the bacteria Vibrio qinghaiensis sp. -Q67

Abstract

Hormesis describes a specific phenomenon in a biphasic concentration-response curve: low concentrations stimulate a response, while high concentrations suppress it. Hormesis could be influenced by several environmental factors, e.g. pH. In this study, the concentration-response/bioluminescence inhibition profiles (CRPs) of six components in personal care products to Vibrio qinghaiensis sp.-Q67 were measured at five different pH levels. When the exposure lasted for 0.25 h, CRPs of the six components at various pH levels were S-shaped, except ascorbic acid 2-glucoside (AA2G) at pH 10.5. When it lasted for 12 h, the CRPs were J-shaped, except AA2G at pH 6.5, 7.5, and 9.5. To rationally explain these changes in hormesis expressed by J-shaped CRP, four characteristic parameters, the minimum effect (Emin) and its corresponding concentration (ECmin), the median effective concentration (EC50), and the zero effect concentration point (ZEP, where the effect is 0 and the concentration is ZEP), were used to quantify the J-shaped CRP. The results indicated that these parameters vary with pH. Additionally, ZEP showed an excellent linear relationship with EC10 (R2 = 0.9994) at all pH levels, indicating that EC10 could replace the no-observed effective concentration (NOEC) in ecological risk assessment. Furthermore, to elucidate the possible mechanism of hormesis, the binding of the components to the luciferase receptors was analyzed using molecular docking technology. The results showed that the components displaying hormesis bind more easily to the α subunit of luciferase than to the β subunit.