Persulfate-based strategy for promoted acesulfame removal during sludge anaerobic fermentation: Combined chemical and biological effects

Abstract

The acesulfame (ACE) degradation in waste activated sludge (WAS) via direct anaerobic fermentation is difficult and the efficient elimination techniques are imperative for the ultimate safe WAS disposal. Persulfate (PS)-based approach was developed to promote the ACE removal during WAS anaerobic fermentation. Results demonstrated the effectiveness of PS-based treatments on ACE degradation, and the ACE removal efficiency was respectively 48.2% and 96.2% in the PS and PS/Fe-treated reactors while it was only 6.0% in the control reactor. Mechanism explorations revealed that the active free radicals (i.e. OH• and SO4•−) generated in the PS-based reactors were the key oxidative species for the ACE degradation. However, such effects were interfered by the released soluble substrates (i.e. protein, carbohydrate and inorganic ions) during anaerobic fermentation by competing and/or quenching free radicals, which caused the deceleration of the ACE removal efficiency. Moreover, the PS-based treatment facilitated the enrichment of functional microorganisms (i.e. Phyllobacteriaceae and Bradyrhizobiaceae) and upregulated the critical genes (i.e. pncB and nadE) involved in the ACE degradation. Based on the density functional theory (DFT) and metabolic intermediates analysis, the hydroxylation and oxidative ring-opening were the two main proposed metabolic pathways for ACE degradation. Overall, the combined chemical and biological metabolism effects collectively contributed to the efficient ACE degradation, and it provided a novel and effective strategy for refractory pollutants removal during WAS anaerobic fermentation.