Abstract
Aim of the work was to study possibility of erythropoiesis stimulation by transplantation of fetal liver cells, seeded into macroporous carriers to the rats with post-hemorrhargic anemia, induced by 70% hepatectomy. Fetal liver cells (FLC) were isolated from fetuses of rats with 15 days’ gestation and were cryopreserved. Decryopreserved FLC were seeded into macroporous spongy alginate-gelatin scaffolds, which were covered by alginate capsule and implanted into omentum of rats with modeled liver insufficiency. It was shown that fetal liver cells, immobilized in macroporous scaffolds after implantation have positive effect on red blood count and hemoglobin content, indicating that this approach is promising for the development of new methods of anemia treatment.
Highlights
Ⱥɧɟɦɢɹ ɹɜɥɹɟɬɫɹ ɨɞɧɢɦ ɢɡ ɧɚɢɛɨɥɟɟ ɪɚɫɩɪɨɫɬɪɚɧɟɧɧɵɯ ɫɢɧɞɪɨɦɨɜ, ɜɫɬɪɟɱɚɸɳɢɯɫɹ ɜ ɩɪɚɤɬɢɤɟ ɜɪɚɱɟɣ ɪɚɡɥɢɱɧɵɯ ɫɩɟɰɢɚɥɶɧɨɫɬɟɣ. ȼ ɩɨɫɥɟɞɧɟɟ ɜɪɟɦɹ ɱɚɫɬɨɬɚ ɡɚɛɨɥɟɜɚɟɦɨɫɬɢ ɚɧɟɦɢɹɦɢ ɜɨɡɪɨɫɥɚ ɜ ɪɟɡɭɥɶɬɚɬɟ ɞɟɣɫɬɜɢɹ ɬɚɤɢɯ ɮɚɤɬɨɪɨɜ, ɤɚɤ ɭɯɭɞɲɟɧɢɟ ɤɚɱɟɫɬɜɚ ɩɢɬɚɧɢɹ, ɫɧɢɠɟɧɢɟ ɨɛɳɟɣ ɪɟɚɤɬɢɜɧɨɫɬɢ ɨɪɝɚɧɢɡɦɚ, ɭɱɚɳɟɧɢɟ ɧɟɪɜɧɵɯ ɫɬɪɟɫɫɨɜ, ɷɤɡɨɝɟɧɧɨɣ ɢ ɷɧɞɨɝɟɧɧɨɣ ɢɧɬɨɤɫɢɤɚɰɢɢ [2]
Ɱɬɨ ɧɟɨɛɯɨɞɢɦɵɦ ɮɚɤɬɨɪɨɦ ɞɥɹ ɷɪɢɬɪɨɩɨɷɡɚ ɹɜɥɹɟɬɫɹ ɷɪɢɬɪɨɩɨɷɬɢɧ, ɜ ɨɬɫɭɬɫɬɜɢɟ ɤɨɬɨɪɨɝɨ ɛɢɨɝɟɧɟɡ ɷɪɢɬɪɨɰɢɬɨɜ ɧɟ ɪɟɚɥɢɡɭɟɬɫɹ. ȿɫɥɢ ɭ ɜɡɪɨɫɥɨɝɨ ɱɟɥɨɜɟɤɚ ɗɉɈ ɜ ɨɫɧɨɜɧɨɦ ɨɛɪɚɡɭɟɬɫɹ ɜ ɩɨɱɤɚɯ, ɬɨ ɜ ɩɪɨɰɟɫɫɟ ɜɧɭɬɪɢɭɬɪɨɛɧɨɝɨ ɪɚɡɜɢɬɢɹ ɝɥɚɜɧɵɦ ɦɟɫɬɨɦ ɟɝɨ ɩɪɨɞɭɤɰɢɢ ɹɜɥɹɸɬɫɹ ɫɬɪɨɦɚɥɶɧɵɟ ɤɥɟɬɤɢ ɩɟɱɟɧɢ [10]. ɉɨɷɬɨɦɭ ɦɨɠɧɨ ɩɪɟɞɩɨɥɨɠɢɬɶ, ɱɬɨ ɩɪɢ ɬɪɚɧɫɩɥɚɧɬɚɰɢɢ ɤɥɟɬɤɢ ɮɟɬɚɥɶɧɨɣ ɩɟɱɟɧɢ ɜ ɭɫɥɨɜɢɹɯ ɞɟɮɢɰɢɬɚ ɷɪɢɬɪɨɰɢɬɨɜ ɦɨɝɭɬ ɜɵɩɨɥɧɹɬɶ ɫɬɢɦɭɥɢɪɭɸɳɭɸ ɪɨɥɶ, ɩɪɨɞɭɰɢɪɭɹ ɗɉɈ ɢ ɞɪɭɝɢɟ ɪɨɫɬɨɜɵɟ ɮɚɤɬɨɪɵ, ɭɫɢɥɢɜɚɸɳɢɟ ɝɟɦɨɩɨɷɡ
Ⱦɥɹ ɩɪɟɞɨɬɜɪɚɳɟɧɢɹ ɩɪɨɧɢɤɧɨɜɟɧɢɹ ɜ ɚɥɶɝɢɧɚɬ-ɠɟɥɚɬɢɧɨɜɵɟ ɝɭɛɤɢ ɤɥɟɬɨɤ ɯɨɡɹɢɧɚ ɢɯ ɩɨɤɪɵɜɚɥɢ ɨɛɨɥɨɱɤɨɣ ɢɡ ɚɥɶɝɢɧɚɬɧɨɝɨ ɝɟɥɹ
Summary
Ⱥɧɟɦɢɹ ɹɜɥɹɟɬɫɹ ɨɞɧɢɦ ɢɡ ɧɚɢɛɨɥɟɟ ɪɚɫɩɪɨɫɬɪɚɧɟɧɧɵɯ ɫɢɧɞɪɨɦɨɜ, ɜɫɬɪɟɱɚɸɳɢɯɫɹ ɜ ɩɪɚɤɬɢɤɟ ɜɪɚɱɟɣ ɪɚɡɥɢɱɧɵɯ ɫɩɟɰɢɚɥɶɧɨɫɬɟɣ. ȼ ɩɨɫɥɟɞɧɟɟ ɜɪɟɦɹ ɱɚɫɬɨɬɚ ɡɚɛɨɥɟɜɚɟɦɨɫɬɢ ɚɧɟɦɢɹɦɢ ɜɨɡɪɨɫɥɚ ɜ ɪɟɡɭɥɶɬɚɬɟ ɞɟɣɫɬɜɢɹ ɬɚɤɢɯ ɮɚɤɬɨɪɨɜ, ɤɚɤ ɭɯɭɞɲɟɧɢɟ ɤɚɱɟɫɬɜɚ ɩɢɬɚɧɢɹ, ɫɧɢɠɟɧɢɟ ɨɛɳɟɣ ɪɟɚɤɬɢɜɧɨɫɬɢ ɨɪɝɚɧɢɡɦɚ, ɭɱɚɳɟɧɢɟ ɧɟɪɜɧɵɯ ɫɬɪɟɫɫɨɜ, ɷɤɡɨɝɟɧɧɨɣ ɢ ɷɧɞɨɝɟɧɧɨɣ ɢɧɬɨɤɫɢɤɚɰɢɢ [2]. Ȼ ɫɜɹɡɢ ɫ ɷɬɢɦ ɤɥɟɬɤɢ ɮɟɬɚɥɶɧɨɣ ɩɟɱɟɧɢ (ɄɎɉ) ɦɨɝɭɬ ɪɚɫɫɦɚɬɪɢɜɚɬɶɫɹ ɜ ɤɚɱɟɫɬɜɟ ɢɫɬɨɱɧɢɤɚ ɷɪɢɬɪɨɰɢɬɨɜ ɩɪɢ ɢɯ ɞɟɮɢɰɢɬɟ. Ɉɨɷɬɨɦɭ ɦɨɠɧɨ ɩɪɟɞɩɨɥɨɠɢɬɶ, ɱɬɨ ɩɪɢ ɬɪɚɧɫɩɥɚɧɬɚɰɢɢ ɤɥɟɬɤɢ ɮɟɬɚɥɶɧɨɣ ɩɟɱɟɧɢ ɜ ɭɫɥɨɜɢɹɯ ɞɟɮɢɰɢɬɚ ɷɪɢɬɪɨɰɢɬɨɜ ɦɨɝɭɬ ɜɵɩɨɥɧɹɬɶ ɫɬɢɦɭɥɢɪɭɸɳɭɸ ɪɨɥɶ, ɩɪɨɞɭɰɢɪɭɹ ɗɉɈ ɢ ɞɪɭɝɢɟ ɪɨɫɬɨɜɵɟ ɮɚɤɬɨɪɵ, ɭɫɢɥɢɜɚɸɳɢɟ ɝɟɦɨɩɨɷɡ.
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