Persistent spectral hole-burning effects in fluorescence line-narrowing spectroscopy. Analytical implications

Abstract

Persistent spectral hole-burning (PSHB) effects on fluorescence line-narrowing (FLN) spectra of tetracene in glassy and polymer matrices have been studied with emphasis on analytical implications. Attention has been paid to the time-evolution of the FLN spectrum under prolonged laser irradiation as well as to the matrix, laser intensity, temperature and excitation wavelength dependence of hole-burning effects. It is shown that PSHB, that generally is an unwanted phenomenon in analytical FLN spectroscopy, can be reduced or completely removed by using a suitable matrix material, by reducing the laser intensity, by working at somewhat elevated temperatures and by employing excitation to the vibronic region of the analyte.