Abstract
Safflower (Carthamus tinctorius L.) is an oil crop species and is widely cultivated since ancient times in Near East. Fifteen peroxidase gene based markers (POGP) were used to fingerprint 39 genotypes (33 cultivars, 6 breeding lines) from 6 countries to asses genetic diversity within elite safflower germplasm. Fourteen POGP markers produced polymorphisms and one marker was monomorphic. POGP markers produced total of 71 bands of which 50 were polymorphic. Average number of bands produced by POGP markers were 4.7, 3.3 of which were polymorphic among the safflower genotypes. To reveal genetic relationships among the safflower genotypes, similarity matrix was calculated and UPGMA method was used to construct a dendrogram. Mean similarity was 0.80 among the genotypes with a range of 0.56-0.91. Genotypes clustered within 2 groups. Gene diversity of the markers ranged between 0.17-0.48. These results show POGP markers could be used to fingerprint to study genetic diversity of safflower genotypes.
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