Abstract
AbstractPeriplasmic binding proteins (PBPs) of gram-negative bacteria have been widely used as recognition elements for the development of biosensors for small molecule analytes owing to their intrinsically high selectivity and affinity towards their cognate ligands. Analyte binding is accompanied by a large hinge motion that can readily be transduced to a detectable signal. While fundamental work demonstrating the versatility of PBPs as scaffolds for biosensors dates back to the 1990s, recent years have seen more subtle improvements in detection strategies. Measurement of cellular metabolites with PBP-based biosensors has allowed significant contributions to basic research, and a first functional sensor for continuous blood glucose monitoring with glucose-binding protein as biological recognition element was tested in preclinical trials. In this chapter, strategies and applications of biosensors using PBPs as specifiers will be reviewed.KeywordsPeriplasmic binding proteinsAffinity based biosensorsSolvatochromismFluorescence biosensors
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