Perioperative changes in intestinal flora of patients underwent laparoscopic colorectal cancer surgery

Abstract

Objective To investigate perioperative changes in intestinal flora of patients underwent laparoscopic colorectal cancer surgery. Methods From June 2016 to December 2017, 46 patients with colorectal cancers were divided into colorectal cancer group, and 46 healthy persons were divided into health examination group. All of 46 patients with colorectal cancers underwent laparoscopic radical surgery. Before and after operation, fresh feces were collected and the DNA of intestinal flora was measured. The intestinal flora was determined by polymerase chain reaction-denaturing gradient gel electrophoresis. Drawing the corresponding map based on the results of the determination. The perioperative changes in intestinal flora were analyzed by using SPSS18.0 software. The diversity and richness of intestinal flora were expressed as (±s), and were examined by using paired t test or F test. A P value<0.05 was considered as statistically significant difference. Results PCR amplification of feces showed that the bands of intestinal microflora were about 200 BP, which coincided with the expected length of DNA fragment. The fecal diversity index was (5.6±0.2) and the richness (E) was (77.1±0.4) in healthy people. Preoperative fecal specimen diversity index (H) was (3.8±0.1), and (4.1±0.1) after operation in colorectal cancer patients. The preoperative abundance (E) was (45.3±0.9), and (50.6+ 0.6) after operation. The diversity and richness of intestinal microflora after operation were higher than those before operation (P 0.05). Conclusion The diversity and richness of intestinal flora in normal people are higher than those in patients with colorectal cancer, which is related to the age and clinical stage of patients. Clinical analysis of intestinal flora is helpfμl to guide clinical treatment. Key words: Colorectal Neoplasms; Laparoscopy; Enterobacteriaceae; Denaturing Gradient Gel Electrophoresis