Changes of intestinal flora in patients with systemic lupus erythematosus

Abstract

Objective To investigate the changes of intestinal flora in patients with systemic lupus erythematosus (SLE) in Heilongjiang province, and to provide some data support for the study of the correlation between SLE and intestinal flora. Methods The feces of 12 patients with SLE and 12 healthy persons were collected.The bacterial DNA was extracted from the fecal samples and the 16s RNA of bacteria was amplified to obtain the sequence of each bacterium by sequencing.Finally, biological information analysis and statistical analysis.SPSS 17 software was used for statistical analysis. Results There were significant differences in intestinal flora between SLE patients and normal subjects, including 2 phylum(P=0.032, 0.029), 2 families(P=0.029, 0.013) and 11 genera(P=0.042, 0.049, 0.047, 0.040, 0.028, 0.039, 0.003, 0.046, 0.048, 0.045 and 0.049). The abundance and phylogenetic diversity of intestinal flora in patients with SLE were significantly lower than those in normal subjects(P 0.05). Conclusions Bacteroidetes and Prevotella may not be suitable for disease diagnosis and differential diagnosis.Ruminococcaceae was a family of microbes which is not affected by regional diet and race.It may play an important role in the development of SLE.Eleven significantly altered genera in SLE patients can be used to distinguish SLE patients from healthy people. Key words: Systemic lupus erythematosus; Intestinal flora; 16S RNA