Abstract
Aim:To investigate the effect of a protein restriction and a supplementation with methyl donor nutrients during fetal and early postnatal life on the expression and epigenetic state of imprinted genes from the IGF system.Materials & methods:Pregnant female rats were fed a protein-restricted diet supplemented or not with methyl donor.Results:Gene expression of the Igf2, H19, Igf1, Igf2r and Plagl1 genes in the liver of male offspring at birth and weaning was strongly influenced by maternal diet. Whereas the methylation profiles of the Igf2, H19 and Igf2r genes were remarkably stable, DNA methylation of Plagl1 promoter was slightly modified.Conclusion:DNA methylation of most, but not all, imprinted gene regulatory regions was resistant to methyl group nutritional supply.
Highlights
Folic acid supplementation is highly recommended at the beginning of pregnancy and, associated with systematic food fortification and/or the use of multivitamins supplements may be responsible for high amounts of methyl donor (MD) micronutrients
The IGFII-receptor, encoded by the Igf2r gene, acts as an antagonist of IGFII function by sequestering IGFII and
Summary
To investigate the effect of a protein restriction and a supplementation with methyl donor nutrients during fetal and early postnatal life on the expression and epigenetic state of imprinted genes from the IGF system.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
