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Abstract
BackgroundIn recent years, skeletal muscle-related ischemia-reperfusion injury (IRI) has become more common. IRI can lead to severe limb injuries, multiple organ failure, and even death in some cases. However, there is still a lack of rapid and sensitive detection methods for IRI in skeletal muscle. This study aims to explore the value of computed tomography perfusion imaging (CTPI) and color-coded digital subtraction angiography (DSA) in assessing acute IRI of skeletal muscle in a rabbit model.MethodsFifty New Zealand white rabbits were randomly assigned to the ischemia-reperfusion (IR) group (n = 40) or sham group (n = 10). After 3 h of surgically-induced hindlimb ischemia, the IR group underwent reperfusion and CTPI and color-coded DSA were taken to assess the skeletal muscle at 0, 6, 12, or 24 h post-reperfusion. The data from CTPI and DSA in the right and left hindlimbs, blood flow (AF-R/L), blood volume (BV-R/L), contrast clearance rate (C-R/L) and the maximum contrast enhancement values (peak-R/L) were obtained. Serum superoxide dismutase (SOD), creatine kinase (CK), lactic dehydrogenase (LDH) and malondialdehyde (MDA) were measured. The statistical correlation between the above parameters (CTPI, color-coded DSA, and biochemical markers) was analyzed.ResultsThe mean value of AF-R/L, BV-R/L, C-R/L and peak-R/L decreased linearly from 1.07 ± 0.08 to 0.75 ± 0.11, 1.03 ± 0.06 to 0.85 ± 0.14, 0.93 ± 0.15 to 0.71 ± 0.18, and 1.07 ± 0.01 to 0.47 ± 0.04, respectively. The correlation coefficients between AF-R/L and SOD, CK, LDH and MDA were 0.57, − 0.44, − 0.60, and − 0.62, respectively (p < 0.001). The correlation coefficients between Peak-R/L and SOD, CK, LDH, MDA were 0.59, 0.68, 0.71 and 0.66, respectively (p < 0.001). The correlation coefficient between AF-R/L and Peak-R/L was 0.70 (p < 0.001).ConclusionBoth CTPI and color-coded DSA could dynamically assess skeletal muscle IRI in rabbits.
Highlights
In recent years, skeletal muscle-related ischemia-reperfusion injury (IRI) has become more common
computed tomography perfusion imaging (CTPI) and color-coded digital subtraction angiography (DSA) were successfully achieved in each group (Figs. 3 and 4)
CTPI and color-coded DSA parameters The detailed CTPI and color-coded DSA parameters are shown in Tables 1 and 2
Summary
Skeletal muscle-related ischemia-reperfusion injury (IRI) has become more common. IRI can lead to severe limb injuries, multiple organ failure, and even death in some cases. IRI involving skeletal muscle has become more common and can lead to severe limb injuries, multiple organ failure, and even death in some cases [3]. IRI can be evaluated by its clinical manifestations, including clinical symptoms, medical imaging, and measurement of certain serum biochemical biomarkers. While providing vital insight into disease severity, the measurement of these biomarkers requires time and may not be feasible during urgent traumas. For this reason, medical imaging had become a key player in the diagnosis and treatment planning of IRI conditions
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